"FRET is a non-radiative transfer of energy from an excited donor fluorophore molecule to a nearby acceptor fluorophore molecule...When the biomolecule of interest is present, it can cause a change in the distance between the donor and acceptor, leading to a change in the efficiency of FRET and a corresponding change in the fluorescence intensity of the acceptor. This change in fluorescence can be used to detect and quantify the biomolecule of interest."
advantages:
real-time
non-destructive
sensitive to very low concentrations (picomolar and nanomolar)
highly specific because it detects conformational changes in biological molecules
this article is from a not-great journal and the author clearly does not have English as a first language... at some point i will need a more reputable source, this was from googling FRET quickly
https://www.astralcodexten.com/p/the-case-against-proposition-36 Clara Collier gives the narrow, evidence-based case that shorter jail sentences didn't cause California's property crime wave or drug overdose death epidemic, and longer jail sentences won't fix those problems
I'm pretty convinced but I don't follow this topic in great detail
metastatic malignant peripheral nerve sheath tumor is pretty bad -- median survival is only 8 months after metastases are detected. but one M.O. that seems to help in several case studies is "sequence the tumor, find a mutation, use a drug that's approved for other cancer types with the same mutation."
PD-L1 overexpression? use a PD-1 inhibitor! checkpoint immunotherapy stays winning.
chemo is...not great but better than nothing. some partial responses, no complete responses, survival extended by maybe a few months. mostly it seems best to have doxorubicin in the mix.
mech-interp seems like straightforwardly real and good work from a variety of perspectives on AI. helps with many risk scenarios including some x-risk scenarios; helps make the technology stronger & more reliable, which is good for the industry in the long run.